Histotechnologist - HTL

Obtain certification and advance your career in histology with our specialized credential.

Performs both routine and complex histologic techniques such as enzyme histochemistry, immunohistochemistry, in situ hybridization, and immunofluorescence on tissue samples for microscopic examination by a pathologist to diagnose disease. Knowledgeable in the areas of pathology, management, education, and regulations that impact the field of histotechnology.

ASCP and ASCPi Pathways

Attaining an American Society for Clinical Pathology Board of Certification (ASCP BOC) credential requires a laboratory professional or other expert to meet specific education, training, and/or experience standards and to successfully pass an examination demonstrating knowledge and applied skills. The ASCP BOC offers ASCP and ASCPi credentials based on distinct eligibility requirements. All candidates must carefully compare credentialing options. Additional information is available about the similarities and differences between similarities and differences between ASCP and ASCPi credentials and ASCPi credentials.

Eligibility Routes

Each credential has specific eligibility requirements, which are different for ASCP and ASCPi. It’s your responsibility to confirm that your education, training, and/or experience meet the criteria. Submit the correct documentation with your application. Once submitted, the category and route of application cannot be changed.

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Histotechnologist – HTL(ASCP)

An applicant must be able to verify that they meet all eligibility requirements, including education, training, and/or experience. For complete information, see the Documentation section of the website. 

Education does not need to be from the United States. Details are available by reviewing the webpage titled Acceptable Education, also available in the routes below. 

To be eligible for this examination category, an applicant must satisfy the minimum requirements (for degrees, the stated degree or higher) of at least one of the following routes:

An applicant must be able to verify that they meet all eligibility requirements, including education, training, and/or experience. For complete information, see the  Documentation section of the website. 

Education needs to be equivalent to education from the United States. Details are available by reviewing the webpage titled  Acceptable Education, also available in the routes below. 

To be eligible for this examination category, an applicant must satisfy the minimum requirements (for degrees, the stated degree or higher) of at least one of the following routes: 

    • Baccalaureate degree from an accredited (regionally or nationally) college/university with a combination of 30 semester hours (45 quarter hours) in biology and chemistry (must include credit hours in both), which may be obtained within, or in addition to, the baccalaureate degree,  
    • AND successful completion of a NAACLS-accredited Histotechnician or Histotechnologist program within the last 5 years. 
    • The education received from a NAACLS-accredited Histotechnician or Histotechnologist program is acceptable for a period of 5 years from the date of completion of that program.
    • AND 1 year of full-time acceptable clinical* experience in a histopathology laboratory within the last 5 years that must be documented on this Experience Documentation Form OR 1 year of full-time acceptable veterinary, industry or research experience in a histopathology laboratory in the U.S. or Canada within the last 5 years that must be documented on this Experience Documentation Form OR completion of a two-part structured histology program of at least 9 months that includes both classroom (didactic) training and a minimum of 480 hours of acceptable clinical* experience in a histopathology laboratory within the last 5 years that must be documented on this Structured Program Documentation Form.


    *Acceptable Clinical Laboratory:
    CMS CLIA certificate of registration, compliance, accreditation;
    OR DoD CLIP certificate of registration, compliance, accreditation;
    OR JCI accreditation;
    OR Accreditation under ISO 15189.

    Experience

    To fulfill the experience requirement for the Histotechnologist examination, you must have experience within the last 5 years in ALL of the following areas:

    Fixation

    • Tissue Identification
    • Parameters (e.g., pH, time, temperature)
    • Reagents
    • Selection, preparation, and use of fixatives for various applications
    • Troubleshooting/problem solving of fixation artifacts

    Processing

    • Selection, preparation, and use of decalcification reagents
    • Selection of appropriate processing methods (e.g., routine histology, immunohistochemistry, cytology)
    • Operation and maintenance of a tissue processor

    Embedding / Microtomy

    • Tissue identification and orientation for embedding
    • Operation and maintenance of an embedding center
    • Microtomy (e.g., paraffin, frozen)
    • Operation and maintenance of a microtome / water bath and cryostat

    Staining

    • Selection of appropriate control material
    • Reagent preparation
    • Operation and maintenance of staining equipment
    • Mounting and coverslipping procedures
    • Identification of tissue structures and their staining characteristics
    • Routine staining (i.e., H&E)
    • Special staining (e.g., carbohydrates and amyloid, connective tissue, microorganisms, pigments and minerals)
    • Immunohistochemistry

    Laboratory Operations

    • Operation, preventive maintenance, and corrective action for equipment
    • Troubleshooting
    • Quality control
    • Application of laboratory safety protocols

     


  • *Acceptable Clinical Laboratory:
    CMS CLIA certificate of registration, compliance, accreditation;
    OR DoD CLIP certificate of registration, compliance, accreditation;
    OR JCI accreditation;
    OR Accreditation under ISO 15189.

    Experience

    To fulfill the experience requirement for the Histotechnologist examination, you must have experience within the last 5 years in ALL of the following areas:

    Fixation

    • Tissue Identification
    • Parameters (e.g., pH, time, temperature)
    • Reagents
    • Selection, preparation, and use of fixatives for various applications
    • Troubleshooting/problem solving of fixation artifacts

    Processing

    • Selection, preparation, and use of decalcification reagents
    • Selection of appropriate processing methods (e.g., routine histology, immunohistochemistry, cytology)
    • Operation and maintenance of a tissue processor

    Embedding / Microtomy

    • Tissue identification and orientation for embedding
    • Operation and maintenance of an embedding center
    • Microtomy (e.g., paraffin, frozen)
    • Operation and maintenance of a microtome / water bath and cryostat

    Staining

    • Selection of appropriate control material
    • Reagent preparation
    • Operation and maintenance of staining equipment
    • Mounting and coverslipping procedures
    • Identification of tissue structures and their staining characteristics
    • Routine staining (i.e., H&E)
    • Special staining (e.g., carbohydrates and amyloid, connective tissue, microorganisms, pigments and minerals)
    • Immunohistochemistry

    Laboratory Operations

    • Operation, preventive maintenance, and corrective action for equipment
    • Troubleshooting
    • Quality control
    • Application of laboratory safety protocols

     

    • Baccalaureate degree from an accredited (regionally or nationally) college/university with a combination of 30 semester hours (45 quarter hours) in biology and chemistry (must include credit hours in both), which may be obtained within, or in addition to, the baccalaureate degree,  

    • AND successful completion of a 50-week U.S. military histotechnician training course# within the last 10 years.


    #Official documentation of your military code (NEC, MOS, AFSC code or 68K), official certificate verifying completion of training, or an official copy of your DD214 form, SRB, or ERB must be uploaded in PDF format as part of completing the online application.


  • *Accredited/approved by a governing regulatory association or Ministry. Countries without a prevalent system of accreditation/approval must have programs/educational institutions approved by an International Advisory Board appointed by the ASCP Board of Certification, or eligibility will be determined by an acceptable transcript evaluation. The foreign degree must be equivalent to a U.S. degree.


  • *Accredited/approved by a governing regulatory association or Ministry. Countries without a prevalent system of accreditation/approval must have programs/educational institutions approved by an International Advisory Board appointed by the ASCP Board of Certification, or eligibility will be determined by an acceptable transcript evaluation. The foreign degree must be equivalent to a U.S. degree.

    #Clinical laboratory accredited by JCI, CAP, under ISO 15189 or authorized by a governing regulatory association or Ministry. Countries without a prevalent system of accreditation must have laboratories approved by an International Advisory Board appointed by the ASCP Board of Certification.

    Experience

    To fulfill the experience requirement for the Histotechnologist examination, you must have experience within the last 5 years in ALL of the following areas:

    Fixation

    • Tissue Identification
    • Parameters (e.g., pH, time, temperature)
    • Reagents
    • Selection, preparation, and use of fixatives for various applications
    • Troubleshooting/problem solving of fixation artifacts

    Processing

    • Selection, preparation, and use of decalcification reagents
    • Selection of appropriate processing methods (e.g., routine histology, immunohistochemistry, cytology)
    • Operation and maintenance of a tissue processor

    Embedding / Microtomy

    • Tissue identification and orientation for embedding
    • Operation and maintenance of an embedding center
    • Microtomy (e.g., paraffin, frozen)
    • Operation and maintenance of a microtome / water bath and cryostat

    Staining

    • Selection of appropriate control material
    • Reagent preparation
    • Operation and maintenance of staining equipment
    • Mounting and coverslipping procedures
    • Identification of tissue structures and their staining characteristics
    • Routine staining (i.e., H&E)
    • Special staining (e.g., carbohydrates and amyloid, connective tissue, microorganisms, pigments and minerals)
    • Immunohistochemistry

    Laboratory Operations

    • Operation, preventive maintenance, and corrective action for equipment
    • Troubleshooting
    • Quality control
    • Application of laboratory safety protocols

     


  • *Accredited/approved by a governing regulatory association or Ministry. Countries without a prevalent system of accreditation/approval must have programs/educational institutions approved by an International Advisory Board appointed by the ASCP Board of Certification, or eligibility will be determined by an acceptable transcript evaluation. The foreign degree must be equivalent to a U.S. degree.

    #Clinical laboratory accredited by JCI, CAP, under ISO 15189 or authorized by a governing regulatory association or Ministry. Countries without a prevalent system of accreditation must have laboratories approved by an International Advisory Board appointed by the ASCP Board of Certification.

    Experience

    To fulfill the experience requirement for the Histotechnologist examination, you must have experience within the last 5 years in ALL of the following areas:

    Fixation

    • Tissue Identification
    • Parameters (e.g., pH, time, temperature)
    • Reagents
    • Selection, preparation, and use of fixatives for various applications
    • Troubleshooting/problem solving of fixation artifacts

    Processing

    • Selection, preparation, and use of decalcification reagents
    • Selection of appropriate processing methods (e.g., routine histology, immunohistochemistry, cytology)
    • Operation and maintenance of a tissue processor

    Embedding / Microtomy

    • Tissue identification and orientation for embedding
    • Operation and maintenance of an embedding center
    • Microtomy (e.g., paraffin, frozen)
    • Operation and maintenance of a microtome / water bath and cryostat

    Staining

    • Selection of appropriate control material
    • Reagent preparation
    • Operation and maintenance of staining equipment
    • Mounting and coverslipping procedures
    • Identification of tissue structures and their staining characteristics
    • Routine staining (i.e., H&E)
    • Special staining (e.g., carbohydrates and amyloid, connective tissue, microorganisms, pigments and minerals)
    • Immunohistochemistry

    Laboratory Operations

    • Operation, preventive maintenance, and corrective action for equipment
    • Troubleshooting
    • Quality control
    • Application of laboratory safety protocols

     

Application Fee (non-refundable): $250

Apply for HTL(ASCP)

Beginning January 1, 2026, application fees for all ASCP certification examinations will increase by $10.

Application Fee (non-refundable): $250

Apply for HTL(ASCPi)

Reduced pricing is available for those educated and residing in low-to-middle income countries

Beginning January 1, 2026, application fees for all ASCPi certification examinations will increase by $10, except for those educated and residing in low-to-middle income countries.

Information About Required Documentation

You must upload the appropriate documentation form when you apply online, verifying that you meet the training and/or experience requirements for your category and route of application. You will also need to submit an acceptable transcript or transcript evaluation verifying that you meet the academic requirements. Gather your documents before submitting your application and application fee.

Apply

Once you have confirmed that you meet the requirements and have gathered your documents, submit your online application.

Coming Soon! Digital Badges

In early 2026, the ASCP BOC will launch digital badging through Credly, a trusted provider that increases the visibility of ASCP BOC credential holders by securely showcasing and sharing professional credentials. Learn more about digital badges.

“ASCP BOC credentials are widely recognized as the gold standard for laboratory professionals, offering personal benefits such as career advancement, raises, and professional growth. However, its true value goes beyond individual success. For me, ASCP BOC credentials serve as proof of my competence and commitment to the highest standards in laboratory medicine. It reassures patients that the work I perform is grounded in thorough expertise and precision, and that they are receiving care from someone fully qualified. Credentialing professionals elevates the entire profession, ensuring that laboratory professionals contribute to reliable, accurate diagnostics that can directly impact patient outcomes.”

- Kathryn Bennett, HTL(ASCP)CM