Specialist in Cytometry - SCYM(ASCP) and SCYM(ASCPⁱ)

This list is intended only as a partial reference source. Its distribution does not indicate endorsement by the American Society for Clinical Pathology Board of Certification (ASCP BOC), nor does the ASCP BOC wish to imply that the content of the examination will be drawn solely from these publications.

Journals

• Arnold, L.W. & Lannigan, J. (2010). Practical Issues in High-Speed Cell Sorting.. Practical Issues in High-Speed Cell Sorting. 51, 1.24.1-1.24.30. https://doi.org/10.1002/0471142956.cy0124s51
• Barsky, L.W., et al. (2016). International Society for Advancement of Cytometry (ISAC) Flow Cytometry Shared Resource Laboratory (SRL) Best Practices. Cytometry. 89(11), 1017-1030. https://doi.org/10.1002/cyto.a.23016
• Cossarizza, A., et al. (2019). Guidelines for the Use of Flow Cytometry and Cell Sorting in Immunological Studies (2^nd ed.). European Journal of Immunology, 49(10), 1457-1973. https://doi.org/10.1002/eji.201970107
• Davis B.H., et al. (2013). Validation of Cell-Based Fluorescence Assays: Practice Guidelines from the International Council for Standardization of Haematology and the International Clinical Cytometry Society. Cytometry Part B: Clinical Cytometry, 84(5), 279-357.
• Ferrer-Font, L., et al. (2021). Panel Optimization for High-Dimensional Immunophenotyping Assays Using Full-Spectrum Flow Cytometry. Current Protocols, 1(9), Article e222. https://doi.org/10.1002/cpz1.222
• Lee, J.A., et al. (2008). MIFlowCyt: The Minimum Information about a Flow Cytometry Experiment. Cytometry. Part A: the Journal of the International Society for Analytical Cytology, 73(10), 926-930. https://doi.org/10.1002/cyto.a.20623
• Tung, J.W., et al. (2007). Modern Flow Cytometry: A Practical Approach. Clinics in Laboratory Medicine, 27(3), 453-468. https://doi.org/10.1016/j.cll.2007.05.001
• Welsh, J.A., et al. (2020). MIFlowCyt-EV: A Framework for Standardized Reporting of Extracellular Vesicle Flow Cytometry Experiments. Journal of Extracellular Vescicles, 9(1), 1713526. https://doi.org/10.1080/20013078.2020.1713526

Texts

General Flow Cytometry

• Hawley, T.S. & Hawley, R.G. (Eds.). (2024). Flow Cytometry Protocols (5^th ed.). New York: Humana Press.
• McCoy Jr., J.P. (Ed.). (2019). Immunophenotyping Methods and Protocols. Humana Press.
• Ormerod, M.G. (2008). Flow Cytometry: A Basic Introduction. Redhill: M. G. Ormerod.

Clinical Flow Cytometry

• CLSI. (2007). Enumeration of Immunologically Defined Cell Populations by Flow Cytometry; Approved Guideline (2^nd ed.). CLSI H42-A2. Clinical Laboratory Standards Institute.
• CLSI. (2021). Validation of Assays Performed by Flow Cytometry (1^st ed.). CLSI H62. Clinical and Laboratory Standards Institute.
• Dorfman, D.M., Karlon, W.J., & Linden, M.A. (2023). Color Atlas of Flow Cytometry. College of American Pathologists.
• McPherson, R.A. & Pincus, M.R. (2021). Henry’s Clinical Diagnosis and Management by Laboratory Methods: Chapters 35 and 46 (24^th ed.). Philadelphia: Elsevier.
• Nguyen, D.T., Diamond L.W., & Braylan, R.C. (2007). Flow Cytometry in Hematopathology: A Visual Approach to Data Analysis and Interpretation (2^nd ed.). Totowa, NJ: Humana Press.
• WHO Classification of Tumours Editorial Board. (2024). WHO Classification of Tumours: Haematolymphoid Tumours (5^th ed., Vol. 11). Lyon, France: IARC Press.

Research Flow Cytometry

• Barteneva, N.S. & Vorobjev, I.A. (Eds.). (2016). Imaging Flow Cytometry: Methods and Protocols. New York: Springer.
• Ortolani, C. (2022). Flow Cytometry Today: Everything You Need to Know about Flow Cytometry. Switzerland: Springer.
• Shapiro, H.M. (2003). Practical Flow Cytometry (4^th ed.). New York: Wiley-Liss.

Laboratory Administration

• U.S. Department of Health and Human Services (2020). Biosafety in Microbiological and Biomedical Laboratories (6^th ed.). HHS. Pulication No. (CDC) 300859.
• Garcia, L.S., et al. (Eds.). (2024). Clinical Laboratory Management (3^rd ed.). Washington D.C.: ASM Press.
• Harmening, D.M. (2020). Laboratory Management: Principles and Processes (4^th ed.). St. Petersburg: D.H. Pub. & Consulting, Inc.

Online

• The Single Cell Omics Group
• Centers for Medicare and Medicaid Services (includes CLIA regulations)
• Clinical and Laboratory Standards Institute (CLSI)
• Clinical Cytometry Education Network (CCEN)
• College of American Pathologists (CAP)
• International Society for Advancement of Cytometry (ISAC) Cyto University
• International Clinical Cytometry Society (ICCS)
  • International Clinical Cytometry Society (ICCS) Quality and Standards Committee
• Occupational Safety & Health Administration (OSHA)

Reviewed July 2025