Debut Presentation!    5195     Flow Cytometry: Immunophenotyping of Hematolymphoid Neoplasia
8:30 am - 4:30 pm            6.5 CMLE Credits

Jayne L. Healey, MD, FASCP
Senior Hematopathologist, Director, Flow Cytometry, Genoptix Medical Laboratory, Carlsbad, CA

Teri A Oldaker, CLS, CC; QCYM(ASCP)
Technical Director, Flow Cytometry, Genoptix Medical Laboratory, Carlsbad, CA

Update your knowledge of this important area of the laboratory with an information-filled discussion of the flow cytometric immunophenotyping of hematolymphoid neoplasms in the clinical laboratory. First you will be introduced to crucial technical concepts such as cellular analysis, flow cytometer design and operation, fluorescence and fluorochromes, sample preparation, instrument compensation, and quality control.  You’ll then participate in a presentation of data acquisition, display, and analysis, with a focus on normal patterns. The immunophenotypic classification of hematolymphoid neoplasms will be the focus of subsequent discussion, followed by a review of case studies to reinforce your application of the practical information presented.

Following this workshop, you will be able to:

• Avoid common errors in preparation, gating and analysis.  
• Recognize data patterns and trends that may signify global technical problems.
• Identify normal and abnormal patterns in lymphocyte immunophenotyping. 
• Explain the immunophenotypic classification of hematopoietic neoplasms.
• Review flow cytometric data for accuracy and precision.

Debut Presentation!      6820        R3-5 for Blood Bankers
8:30 am - 4:30 pm     6.5 CMLE Credits

Teresa Y. Harris, MT(ASCP)SBB, CQIA(ASQ)
Emerita, Operational Support for Immunohematology Laboratories, American Red Cross; Independent Consultant, Winthrop, WA

This intermediate workshop will focus on of the key elements (R³) of blood banking (1) Regulations (2) Review of blood groups and other Reactivity that might be seen in blood banking, and (3) Resolution of Reactivity by using tools available in the blood banker’s toolbox. The workshop will focus on participants who may routinely work in the blood bank, rotate through the blood bank, or who have been away from the blood bank for a while. Students will also benefit from this overview of blood banking, which is focused on staff from hospitals wanting to expand their knowledge of regulations, blood groups, and techniques. Case studies will expand your knowledge and experience and illustrate the resolution of unexpected serological reactivity.  This session will review and contrast the methods used in today’s transfusion services. While component therapy will not be the focus of the workshop, transfusion strategies for case studies will be discussed.

Following this workshop, you will be able to:  

• Describe current standards and regulations and how they apply to the transfusion service.
• Discuss the common and uncommon blood group systems and collections.
• Analyze serological and clinical data in order to resolve problems commonly encountered in the transfusion service.
• Evaluate serologic data to select appropriate tools for serologic resolution and blood for transfusion.  

7829   Show Me the Diagnosis! Case Presentations in Clinical Microbiology
8:30 am - 4:30 pm   6.5 CMLE Credits

Joseph M. Campos, PhD
Director, Microbiology Laboratory and Laboratory Informatics, Children's National Medical Center; Professor, Departments of Pediatrics, Pathology and Microbiology/Immunology, George Washington University Medical Center, Washington, DC

A. Beth Prouse, MS, MT(ASCP)
Clinical Microbiologist, Peninsula Regional Medical Center, Salisbury, MD

Meet the daily challenges in microbiology: a patient with an infectious disease can be a challenging mystery. Specimen handling test selection, and specimen workup decisions are based on many scraps of clinical and laboratory information that, when considered alone, may be of little diagnostic value.  Clinical microbiologists, more so than other laboratorians, must function like detectives, gathering the evidence necessary for a correct etiologic diagnosis. 
 
Enhance your microbiology knowledge and sharpen your diagnostic abilities, as you resolve the infectious disease cases presented. Your interaction reinforces the information presented as unknown bacterial, mycobacterial, fungal, parasitic, and viral etiologies are assessed.  Following the case studies, hear a thorough discussion on the infectious agents involved.  Incorporating both clinical and laboratory standpoints, the workshop emphasizes accurate, cost-effective laboratory workup procedures.

Following this workshop, you will be able to:  

• Recognize the clinical syndromes associated with common presentations of bacteremia, meningitis, pneumonia, urinary tract infection, sexually transmitted and other infectious diseases.
• Cite the laboratory methods and procedures that are most appropriate for the accurate identification of the pathogens, stressing cost-effective approaches.

“I benefitted from the whole experience from lecturers to peers. Very informative and a confidence booster.”