Tuesday


5782     Peripheral Smears: The Primary Diagnostic Tool Part I: The Myeloproliferative Disorders
8:00 am – 4:00 pm     7.0 CMLE Credits

Irma Pereira, MT(ASCP)SH
Emerita, Clinical Hematology Specialist and Consultant, Clinical Hematology; Adjunct Lecturer in Hematology, Department of Medicine, Stanford University Hospital, Stanford, California

Although this workshop is Part I of two sessions, each may be taken separately.  Part II will be presented on June 12.

Diagnostic ability is essential for the proper evaluation of a patient's hematological status. Erroneous or inaccurate results can lead to an incorrect diagnosis, causing delayed or inappropriate treatment. Yet each day, you are called upon to analyze cell morphology on peripheral smears without the benefit of a case history, in a rather "blind study" fashion. Attend this intermediate-level workshop to increase your hematological acuity and gain self-assurance in your morphology and diagnostic skills! In this presentation, you will:

  • view more than four hundred images demonstrating techniques for the peripheral smear diagnosis of myeloproliferative disorders
  • discuss differential diagnosis exercises, using only peripheral smear findings, to challenge your knowledge and reinforce the information presented
  • hear in-depth presentations on:  
    • chronic Myeloproliferative Disorders (CML, PV, CIMF, ET, CNL) and early transformation recognition
    • Myelodysplastic Syndromes and the CMPD/MDS Syndromes (CMML, JMML, aCML)
    • WHO classification of the myelodysplastic disorders (RA, RARS, RAMLD, RAEB [types l and ll] and 5q- Syndrome)
    • acute Myeloid Leukemias according to WHO Classification, including the new sub-classifications vs. the renamed
    • FAB classification (AML NOS)
    • granulocyte dysfunction syndromes

Following this workshop, you will be able to:
  • More accurately diagnose hematologic problems through improved morphologic skills.
  • Guide non-hematologist physicians to a clearer understanding of patient disorders.
  • Alert physicians to unknown hematologic situations.
  • Discuss patient diagnoses at a higher technical level.


6701     Train for Change: Build Your Knowledge, Comprehension, and Confidence in Molecular Diagnostics
8:00 am - 3:30 pm  6.5 CMLE Credits

Catherine L. Gebhart, PhD, MB(ASCP)CM
Technical Director Molecular Diagnostics Laboratory, The Nebraska Medical Center; Assistant Professor, University of Nebraska Medical Center, Omaha, NE

Get ready! Laboratories of all sizes are adding molecular diagnostic methods to their testing menus, across multiple laboratory sections. If you need an introduction to high complexity molecular diagnostics or a comprehensive overview across multiple molecular diagnostic disciplines, this workshop is for you! The presentation will begin with a review of fundamental concepts and techniques, including hybridization, enzyme restriction, PCR, and Sanger sequencing, followed by discussion of specialty-specific considerations (e.g. infectious disease vs. genetics) for specimen requirements, processing, test methods, quality control, and data interpretation. You will have opportunities to interpret actual test results and troubleshoot problem scenarios for a variety of platforms.

Following this workshop, you will be able to:

  • Describe fundamental molecular diagnostic concepts and techniques.
  • Discuss specialty-specific sample requirements, extraction and test methods, and quality control for molecular testing.
  • Design, implement, and monitor appropriate quality control measures for molecular tests.
  • Evaluate run data and recognize and troubleshoot commonly encountered problems for a variety of testing platforms.
  • Select, validate, and maintain molecular tests for your laboratory, using appropriate parameters.






6825    Blood Banking for the Generalist
8:00 am - 3:30 pm    6.5 CMLE Credits

Jack A. Hager, MS, MT(ASCP)SBB
CEO, American Red Cross National Testing Laboratory, Portland, OR

Knowledge and skill with blood typing and antibody detection and identification are essential in transfusion services and donor centers to avoid mistakes or delays that can adversely affect patient care. But, as you rotate through the blood bank, are you unsure of the steps to take if you get a front and back type discrepancy or a positive antibody screen on a patient who has STAT blood bank needs? Do you need confidence when handling a call from the emergency room or surgery for results or products before the problems are fully resolved? Then, this workshop is for you! Specifically designed for laboratory professionals who need a blood bank refresher to upgrade their skills and become more valuable in the workplace, this presentation will focus on performing and interpreting front and back types, antigen screening, and antibody identification. The most prevalent RBC antigens and antibodies will be discussed to give you the knowledge and proficiency you need to increase your confidence to work in the blood bank, make decisions regarding type discrepancies and positive antibody screens, and deal with issuing non-type specific products or those that are not fully compatible. Exercises and case studies will reinforce the information presented.

Following this workshop, you will be able to:

  • Correctly apply the principles of the ABO system, discuss common variations of antigens and antibodies of the ABO system, identify and investigate common front/back type discrepancies and help guide physicians in selection of both type specific and type compatible products.
  • Discuss the Rh System antigens and phenotype prevalence, and determine how and when to proceed with antigen screening and selection of antigen typed RBC to avoid sensitization.
  • Distinguish clinically significant from clinically insignificant antibodies to other common blood group antigens, perform antibody screening and routine antibody identification and evaluate methods for antigen screening and cross match.


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