Freida L. Carson, PhD, HT(ASCP)
Department of Pathology (retired), Baylor University Medical Center, Dallas, TX

Christa Hladik, AA, HT(ASCP)^cm, QIHC
Neuropathology and Immunohistochemistry, UT Southwestern Clinical Laboratories, University of Texas Southwestern Medical Center at Dallas, TX

Keywords: light microscope, polarizing microscope, darkfield microscope, phase-contrast microscope, fluorescence microscope, electron microscope

Upon completion of this activity, you will be able to:

• describe the types of microscopy and identify indications for their use.
• state the most common objectives used in light microscopy and the approximate magnification of each.
• describe the method of determining total magnification.
• define resolution, ocular, objective, micrometry, achromatic, apochromatic, binocular, and parfocal.
• describe routine microtome maintenance.