D. Robert Sutherland, MSc
Department of Laboratory Hematology, University Health Network, Toronto, Canada

Nancy Kuek
Department of Laboratory Hematology, University Health Network, Toronto, Canada

Juan Azcona-Olivera, PhD
R&D Systems, Minneapolis, MN

Tanya Anderson
Department of Laboratory Hematology, University Health Network, Toronto, Canada

Erica Acton
Department of Laboratory Hematology, University Health Network, Toronto, Canada

David Barth, MD
Department of Laboratory Hematology, University Health Network, Toronto, Canada

Michael Keeney
London Health Sciences Centre, London, Canada

Keywords: Fluorescent aerolysin assay; FLAER assay; Flow cytometry; Paroxysmal nocturnal hemoglobinuria clones

Upon completion of this activity, you will be able to:

• discuss the role of the fluorescent aerolysin (FLAER)-based assay in the detection of paroxysmal nocturnal hemoglobinuria (PNH) clones.
• compare the sensitivity of the FLAER assay in WBCs vs the CD59 assay on RBCs in screening for PNH.
• list the technical issues involved in the screening of RBCs for PNH clones.