Hiep Phuc Dong, MSc
Division of Pathology, Norwegian Radium Hospital, Oslo University Hospital, Oslo, Norway

Arild Holth, BSc
Division of Pathology, Norwegian Radium Hospital, Oslo University Hospital, Oslo, Norway

Lilach Kleinberg, PhD
Division of Pathology, Norwegian Radium Hospital, Oslo University Hospital, Oslo, Norway

Marit Gunhild Ruud, BSc
Division of Pathology, Norwegian Radium Hospital, Oslo University Hospital, Oslo, Norway

Mari Bunkholt Elstrand, MD
Section for Gynecologic Oncology, Division of Obstetrics and Gynecology, Norwegian Radium Hospital, Oslo University Hospital, Oslo, Norway

Claes G. Tropé, MD, PhD
Section for Gynecologic Oncology, Division of Obstetrics and Gynecology, Norwegian Radium Hospital, Oslo, and Faculty Division Radiumhospitalet, the Medical Faculty, University of Oslo

Ben Davidson, MD, PhD
Division of Pathology, Norwegian Radium Hospital, Oslo University Hospital, Oslo, Norway, and Faculty Division Radiumhospitalet, the Medical Faculty, University of Oslo

Björn Risberg, MD, PhD
Division of Pathology and Institute for Medical Informatics, Norwegian Radium Hospital, Oslo University Hospital, Oslo, Norway

Keywords: Phosphatidylserine; Ovarian carcinoma; Effusions; Cell surface; Annexin-V; Apoptosis; Flow cytometry

Upon completion of this activity, you will be able to:

• explain the principle of the flow cytometry (FCM) annexin-V apoptosis assay.
• describe the site of measurement of phosphatidylserine on apoptotic cells.
• compare the performance of the annexin-V assay with the other methods used for measuring apoptosis.
• describe how to identify epithelial cells in FCM analysis.